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Sreenivas, S. S.
- Evaluation of Trichoderma viride formulations against Sore Shin Disease in Flue-Cured Virginia (FCV) Tobacco Nurseries
Abstract Views :280 |
PDF Views:181
Authors
Affiliations
1 Department of Molecular Biology, Yuvaraja's College, University of Mysore, Mysore 570 005, Karnataka, IN
2 Central Tobacco Research Institute, Hunsur, Karnataka, IN
1 Department of Molecular Biology, Yuvaraja's College, University of Mysore, Mysore 570 005, Karnataka, IN
2 Central Tobacco Research Institute, Hunsur, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 4 (2011), Pagination: 333-336Abstract
Studies were conducted during 2009–10 to 2010–11 to evaluate talc and neem cake formulations of Trichoderma viride for controlling sore shin disease in FCV tobacco nurseries incited by Rhizoctonia solani. The pooled data analysis of two years indicated that T. viride in neem cake formulation was more effective than the talc formulation and reduced sore shin disease by 63.53%. This effective bio-intensive module reflected on the better yield of healthy transplants (835 m−2) as against untreated (489 m−2) check. The schedule gave an Incremental Cost Benefit Ratio (ICBR) of 1:10.27.Keywords
Rhizoctonia solani, FCV Tobacco, Hore Shin, Trichoderma viride.References
- Amal, A. A., Abd-Elsalam, K. A., Omar, M. R. and Aly, A. A. 2005. Antagonistic potential of Trichoderma spp. against Rhizoctonia solani and use of M13 microsatellite-primed PCR to evaluate the antagonist genetic variation. Journal of Plant Diseases and Protection, 112: 550–561.
- Devaki, N.S. 1991. Studies on Pythium and Phytophthora on tobacco in Karnataka. Ph.D. Thesis. University of Mysore, Mysore, India, 165 p.
- Elad, Y. and Chet, T. 1983. Improved selective media for isolation of Trichoderma or Fusarium spp. Phytoparasitica, 11: 56–58.
- Elad, Y., Chet, I. and Katan, J. 1980. Trichoderma harzianum – a biological agent effective against Sclerotium rolfsii and Rhizoctonia solani. Phytopathology, 70: 119–121.
- Gopalachari, N. C. 1984. Tobacco. Indian council of Agricultural Research, New Delhi, 327 p.
- Mahalakshmi, K., Indra Hooda. and Karwasra, S. S. 2008. Effect of pre-plant herbicides on the biocontrol efficacy of Trichoderma spp. against Rhizoctonia solani. Journal of Cotton Research and Development, 22: 221–224.
- Mathivanan, N., Prabavathy, V. R. and Vijayanandraj, V. R. 2005. Application of talc formulations of Pseudomonas fluorescens Migula and Trichoderma viride Pers. ex S.F. Gray decrease the sheath blight disease and enhance the plant growth and yield in rice. Journal of Phytopathology, 153: 697–701.
- Nawar, L. S. 2007. Pathological and rhizospherical studies on ischolar_main-rot disease of squash in Saudi Arabia and its control. African Journal of Biotechnology, 6: 219–226.
- Ramakrishnan, G., Jeyarajan, R. and Dinakaran, D. 1994. Talc based formulation of Trichoderma viride for biocontrol of Macrophomina phaseolina. Journal of Biological Control, 8: 41–44.
- Saikia, M. K. and Gandhi, S. K. 1995. Comparative activities of three antagonistic fungi against cauliflower stem rot pathogen Rhizoctonia solani. Journal of Mycology and Pathology, 33: 138–140.
- Shenoi, M. M., 1998. Flue Cured Virginia tobacco production package for Karnataka. CTRI (ICAR) Res. Stn., Hunsur, 46 p.
- Shenoi, M. M. 2010. Annual report. Central Tobacco Research Institute, ICAR, Rajahmundry, Andhra Pradesh, 112 p.
- Shenoi, M. M. and Nagarajan, K. 2000. Diseases of FCV tobacco and their management in Karnataka light soil region. Central tobacco research institute, Rajmundry, (Indian council of Agricultural Research), CTRI Research station, Hunsur, Karnataka, India, 31 p.
- Shenoi, M. M. and Sreenivas, S. S. 2007. Bio-intensive integrated disease management of FCV tobacco nursery in Karnataka Light soil. Journal of Biological Control, 21: 197–202.
- Susan, M. B. 1999. Innovative applications of microbial agents for biological weed control, pp.73-77. In: Mukerji, K. G., Chamola, B. P. and Upadhyay, R. K. (Eds). Biotechnological approaches in biocontrol of plant pathogens. Kluwer Academic Publishers, New York.
- Thiruvudainambi, S., Chandrasekar, G. and Baradhan, G. 2010. Management of stem-rot (Sclerotium rolfsii Sacc.) of groundnut through non-chemical methods. Plant Archives, 10: 633–635.
- Wani, A. H., Taskeen-un-nisa. and Mir, R. A. 2009. Biological control of Fusarium oxysporum f. sp. lycopercici on tomato with some fungal antagonists. Journal of Biological Control, 23: 169–173.
- Bio-Management of Fusarium Wilt Disease Complex with Pseudomonas fluorescens and Aspergillus niger
Abstract Views :334 |
PDF Views:96
Authors
Affiliations
1 Central Tobacco Research Institute Research Station, Hunsur 571 105, Karnataka, IN
1 Central Tobacco Research Institute Research Station, Hunsur 571 105, Karnataka, IN
Source
Journal of Biological Control, Vol 26, No 4 (2012), Pagination: 368-372Abstract
Flue Cured Virginia (FCV) tobacco is a major rain-fed crop grown in light soil regions of Karnataka. Fusarium wilt disease complex caused by Fusarium oxysporum f. sp. nicotianae in association with Root-knot nematodes, Meloidogyne incognita is a major threat to the successful production and also for its sustainability in the region. The tobacco crop affected with above malady exhibit stunted growth, severe wilt symptoms, drying of leaves on one side of the plant and with conspicuous galls on the infected ischolar_mains resulted in heavy yield and quality loss. Fungicides and nematicides are being used against this wilt disease complex with limited efficacy. Extensive use of pesticides of chemical origin especially in higher doses for disaese control has to be avoided due to higher costs and associated hazards to the environment. Replicated trials were conducted with antagonistic bacterium, Pseudomonas fluorescens and antagonistic fungi, Aspergillus niger singly and in combinations against fusarium wilt disease complex in FCV tobacco under sick field conditions. Results revealed that application of P. fluorescens @ 1g/plant in combination with A. niger enriched with FYM @ 100 g /plant at the time of planting resulted in 61.0% reduction in fusarium wilt disease incidence at 70 DAT compared to untreated check. There was significant reduction in ischolar_main knot nematode incidence in terms of RKI (Root- Knot Index) to 1.93 and final soil nematode population to 72.5 as compared to RKI of 3.71 and final soil nematode population of 140 in untreated check. Subsequent increase in total cured leaf and bright grade yield was 1311 kg/ha and 926 kg/ha respectively as compared to 1042 kg/ha and 615 kg/ha respectively in untreated check.Keywords
Pseudomonas fluorescens, Aspergillus niger, FusariumWilt Complex, Meloidogyne incognita, FCV Tobacco.References
- Barua L, Bora BC. 2008. Comparative efficacy of Trichoderma harzianum and Pseudomonas fluroscens against Meloidogyne incognita and Ralstonia solanacearum complex in brinja. Indian J Nematol. 38: 86–89.
- Gomez KA, Gomez AA. 1984. Statistical procedure for Agricultural Research. John Wiley and Sons, New York, USA. 680 p.
- Hussaini SS. 1983. Quantification of ischolar_main knot nematode damage on FCV tobacco. Tobacco Res. 9: 61–65.
- Kavitha J, Jonathan EI, Umamaheswari R. 2007. Field application of Pseudomonas fluroscens, Bacillus subtilis and Trichoderma viride for the control of Melodogyne incognita (Kofoid white) chitwood on sugarbeet. J Biol Control 21: 211–215.
- Mani MP, Sivakumar CV. 1998. Management of potato cyst nematode, Globodera spp. through plant rhizosphere bacteria, Pseudomonas fluroscens. J Biol Control 12: 131–134.
- Powel NT. 1971. Interactions between nematodes and fungi in disease complexes. Ann Rev Phytopathol. 9: 253–274.
- Ramakrishnan S, Sreenivas SS, Viswanath SM. 2001. Community structure of plant parasitic nematodes associated with FCV tobacco in KLS region. Tobacco Res. 27: 79–82.
- Ramakrishnan S, Shenoi MM, Sreenivas SS. 2008. Influence of ischolar_main knot nematode, Meloidogyne spp. on fusarium wilt disease of tobacco. Tobacco Res. 34: 91–92.
- Ramakrishnan S, Sreenivas SS, Shenoi MM. 2009. Influence of antagonistic bacterium, Pseudomonas fluorescens against ischolar_main knot nematode in FCV tobacco nursery. Tobacco Res. 35: 44–45.
- Santhi A, Sivakumar CV. 1995. Biocontrol potential of Pseudomonas fluroscens against ischolar_main-knot nematode, Meloidogyne incognita (Kofoid White, 1919) Chitwood 1949 on tomato. J Biol Control 9: 113–115.
- Shenoi MM. 1998. Flue cured tobacco production package for Karnataka, CTRI (ICAR) Research Station, Hunsur, Karnataka, India, pp 26.
- Shenoi MM, Sreenivas SS, Subrahmanya KN. 2004. Studies on Fusarium wilt in FCV tobacco crop of Karnataka light soils. Tobacco Res. 30: 27–32.
- Shenoi MM, Sreenivas SS. 2007. Bio-intensive integrated disease management of FCV tobacco nursery in Karnataka Light Soils. J Biol Control 21: 197–201.
- Sobita Devi L, Pandey RC. 2001. Bio-agents for the management of Meloidogyne incognita and Fusarium oxysporum f.sp. ciceri on chickpea (Abstract), pp. 147–148. National Congress on Centenary of Nematology in India: Appraisal and future plans. IARI, New Delhi, India. 5th – 7th December, 2001.
- Bio-Intensive Integrated Disease Management of FCV Tobacco Nursery in Karnataka Light Soils
Abstract Views :246 |
PDF Views:134
Authors
Affiliations
1 Central Tobacco Research Institute, Research Station, Hunsur- 571105, Karnataka, IN
1 Central Tobacco Research Institute, Research Station, Hunsur- 571105, Karnataka, IN
Source
Journal of Biological Control, Vol 21, No 2 (2007), Pagination: 197-201Abstract
A commercial bio-pesticide, "Kalisena" containing Aspergillus niger (strain AN 27 of IARI) was evaluated as seed pelleting and in slurry form. The results indicated that Katisena in slurry form was effective and feasible giving 100% control of damping off, 85% control of blight and 90% control of black shank diseases in FCV tobacco nursery. The results are comparable to recommended chemical schedule of Ridomil MZ 72 WP. This effective bio-intensive module reflected on the better yield of healthy transplants (912/sq, m.) as against the recommended Ridomit MZ schedule and untreated (846 and 562 per sq. m.) check, respectively. The schedule gave an incremental cost-benefit ratio of 1: 8.2.Keywords
Aspergillus niger, Integrated Disease Management, Kalisena, Soil Solarization, Tobacco Nursery.- Biological Control of Soil-Borne Fungal and Root-Knot Nematode Disease Complex in FCV Tobacco Nursery
Abstract Views :265 |
PDF Views:102
Authors
Affiliations
1 ICAR-CTRI Research Station, Hunsur-571105, Karnataka, IN
1 ICAR-CTRI Research Station, Hunsur-571105, Karnataka, IN
Source
Journal of Biological Control, Vol 29, No 4 (2015), Pagination: 203-206Abstract
Farm yard manure fortified with bio-agents Pseudomonas fluorescens, Trichoderma asperellum and Aspergillus niger either singly or in rational combinations were evaluated against soil- borne fungal pathogens in FCV tobacco nursery. Results of the trial indicated that, application of P. fluorescens and A. niger fortified FYM @ 4 kg/ m2 recorded 40.2 per cent increase in number healthy transplants (640/ m2) and was on par with recommended chemical schedule (627.5/ m2). At 60 DAS, P.fluorescens + A. niger fortified FYM, P. fluorescens + T. viride fortified FYM and chemical check were on par with each other in recording reduced RKI of 1.97, 2.08 and 1.91 respectively compared to 3.80 as RKI in untreated check. Similarly, Bio-agents fortified FYM recorded significant decrease in damping off at 35 DAS (41.1 to 52.4%), damping off + blight at 45 DAS (44.1 to 52.9%) and black shank (45.7 to 58.3 %) compared to untreated check. Though, the chemical schedule was superior in decreasing the damping off by 90.0%, damping off + blight by 93.9% and black shank by 93.4% compared to untreated check the study infers the effectiveness of bio-agents for reducing the chemical use to minimize environmental pollution.Keywords
Aspergillus niger, FCV Tobacco, Nursery, Pseudomonas fluorescens, Soil-Borne Fungal Diseases, Trichoderma viride.References
- Abdul Wajid SM, Shenoi MM, Sreenivas SS. 1995. Seed bed solarization as a component of integrated disease management in VFC tobacco nurseries of Karnataka. Tobacco Research 21:58-65.
- Shenoi MM, Nagarajan K. 2000. Diseases of FCV tobacco and their management in KLS region. CTRI (ICAR) Research Station, Hunsur.31p.
- Hussaini SS. 1983. Quantification of ischolar_main-knot nematode (Meloidogyne spp.) damage on FCV tobacco. Tobacco Research 25: 61-65.
- Ramakrishnan S, Hussaini SS, Viswanath SM, Shenoi MM. 1998. Effect of basamid G for the control of ischolar_main-knot nematode in FCV tobacco nursery. p 121-124. In: Dhawan SC, Kaushal KK. (eds). Proceedings of the National Symposium on Rational approaches in nematode management for sustainable agricultutre, GAU, Anand.
- Jaykumar J, Ramakrishnan S, Rajendran G. 2007. Suppression of cotton reniform nematode Rotylenchus reniformis with Pseudomonas fluorescens. SAARC J Agric. 5:91-93.
- Shenoi MM, Sreenivas SS. 2007. Bio-intensive integrated disease management of FCV tobacco nursery in Karnataka Light Soils. J Biol Control 21(2):197-201.
- Sriram S, Savithai MJ, Rohini HS, Jalai SK. 2013. The most widely used fungal antagonist for plant disease management in India, Trichoderma viride is Trichoderma asperellum as confirmed by oligonucleotide barcode and morphological characters. Curr Sci. 104(10): 1332.
- Ramakrishnan S, Shenoi MM, Sreenivas SS. 2009. Influence of antagonistic bacterium Pseudomonas fluorescens against ischolar_main-knot nematodes in FCV tobacco nursery. Tobacco Research 35: 44-50.
- Ramakrishnan S, Rajendran S. 2010. Evaluation of Pseudomonas fluorescens and Trichodema viride as seed and soil treatment against ischolar_main-knot nemaotode, Meloidogyne arenaia infecting groundnut. J Biol Control 24: 164-167.
- Ramakrishnan S, Sreenivas SS. 2012. Bio-management of Fusarium wilt disease complex with Pseudomonas fluorescens and Aspergillus niger. J Biol Control 26: 368-372.
- Ramakrishnan S, Panduranga Rao C. 2013. Evaluation of Paecilomyces lilacinus for the management of ischolar_main-knot nematode, Meloidogyne incognita in FCV tobacco nursery. Indian J Nematol. 43: 65-69.
- Seema M, Ramakrishnan S, Sreenivas SS, Devaki NS. 2011. Evaluation of Trichoderma viride formulations against sore shin disease in Flue-Cured Virginia (FCV) tobacco nurseries. J Biol Control 25(4): 333-336.